Precise nucleotide sequence modifications with bidirectionally cleaving class-IIS excision linkers

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.3K citations · Full text

Life Sciences, Microbiology, Molecular Biology +10

Mutagenesis at a specific position in a DNA sequence.

Clyde A. Hutchison, Sierra Phillips, Marshall H. Edgell et al. · Journal of Biological Chemistry · 1978 · 521 citations · Full text

Life Sciences, Microbiology, Molecular Biology +15

In vitro mutagenesis of a circular DNA molecule by using synthetic restriction sites.

Fred Heffron, Magdalene So, Brian J. McCarthy · Proceedings of the National Academy of Sciences · 1978 · 199 citations · Full text

Life Sciences, Genetics, Molecular Genetics +15

Identification of functional regions in the transforming protein of Fujinami sarcoma virus by in-phase insertion mutagenesis

James C. Stone, Tom Atkinson, Michael J. Smith et al. · Cell · 1984 · 113 citations

Life Sciences, Genetics, Molecular Biology +16

Universal restriction endonucleases: designing novel cleavage specificities by combining adapter oligodeoxynucleotide and enzyme moieties

Waclaw Szybalski · Gene · 1985 · 90 citations

Life Sciences, Biotechnology, Molecular Biology +15