Studies on the Mechanism of Human Red Cell Loss of Viability during Storage at +4°C in vitro.

Abstract

Red cells stored in SAGM medium for 42 days at +4 degrees C were rejuvenated by bicarbonate, pyruvate and adenosine. Autologous 24-hour posttransfusion survival was determined in untreated as well as rejuvenated cells and showed an improvement from 77.4 +/- 4.7 to 89.2 +/- 7.2%. The erythrocyte adenylate energy charge decreased relatively more than the total adenylate concentration during storage, but the latter correlated better with posttransfusion red cell survival. Considerable deteriorations in red cell morphology (expressed as morphology index) and in deformability (measured as red cell fluidity) were observed during storage but were partly reversed by rejuvenation. The morphology index and the posttransfusion survival showed a significant correlation (r = 0.95, p less than 0.005) after, but not before, rejuvenation, indicating that the remaining changes are more permanent and decisive of survival. It is suggested that, in the proportion of stored erythrocytes which respond to rejuvenation, the capacity and time dependence of recovery of normal shape and flexibility are important.