Preparative in vitro mRNA synthesis using SP6 and T7 RNA polymerases

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Ulrich K. Laemmli · Nature · 1970 · 251.6K citations

Life Sciences, Microbiology, Molecular Biology +14

Efficient<i>in vitro</i>synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

Douglas A. Melton, Paul A. Krieg, Michael R. Rebagliati et al. · Nucleic Acids Research · 1984 · 6.4K citations · Full text

Life Sciences, Biotechnology, Molecular Biology +11

Purification of Mouse Immunoglobulin Heavy‐Chain Messenger RNAs from Total Myeloma Tumor RNA

Charles Auffray, François Rougeon · European Journal of Biochemistry · 1980 · 2.7K citations · Full text

Life Sciences, Biochemistry, Molecular Biology +12

Functional messenger RNAs are produced by SP6<i>in vitro</i>transcription of cloned cDNAs

Paul A. Krieg, Douglas A. Melton · Nucleic Acids Research · 1984 · 1.5K citations · Full text

Life Sciences, Genetics, Molecular Biology +17

A Continuous Cell-Free Translation System Capable of Producing Polypeptides in High Yield

Alexander S. Spirin, Vladimir Baranov, Lyubov A. Ryabova et al. · Science · 1988 · 602 citations

Life Sciences, Biotechnology, Molecular Biology +11