Phytoalexin Accumulation in <i>Arabidopsis thaliana</i> during the Hypersensitive Reaction to <i>Pseudomonas syringae</i> pv <i>syringae</i>

Abstract

Inoculation of leaves of Arabidopsis thaliana (L.) Heynh. with the wheat pathogen, Pseudomonas syringae pv syringae, resulted in the expression of the hypersensitive reaction and in phytoalexin accumulation. No phytoalexin accumulation was detected after infiltration of leaves with a mutant of P. s. syringae deficient in the ability to elicit a hypersensitive reaction; with the crucifer pathogen, Xanthomonas campestris pv campestris; or with 10 millimolar potassium phosphate buffer (pH 6.9). Phytoalexin accumulation was correlated with the restricted in vivo growth of P. s. syringae. A phytoalexin was purified by a combination of reverse phase flash chromatography, thin layer chromatography, followed by reverse phase high performance liquid chromatography. The Arabidopsis phytoalexin was identified as 3-thiazol-2'-yl-indole on the basis of ultraviolet, infrared, mass spectral, (1)H-nuclear magnetic resonance, and (13)C-nuclear magnetic resonance data.