An enzyme electrode for determination of aromatic diamines and aminophenols based on cross-linked ceruloplasmin

Abstract

The determination of aromatic amines and phenols by an enzyme pO 2 electrode containing cross-linked ceruloplasmin in the reaction layer was examined. The electrode shows at pH 5.5 a marked selectivity for derivatives with-NH 2 or NR 2 , resp., and -OH-groups in para and ortho position while its sensitivity for aromatic diamines is better than its sensitivity for aminophenols and polyphenols. p -Phenylenediamine and its N,N-dialkyl derivatives are the best analytes which can be detected starting from concentrations of 10 -5 mol l -1 and higher; the reproducibility is 5-7% at an electrode response time of 2-5 min.