Investigation of Reactivity and Selectivity of DNA‐Alkylating Duocarmycin Analogues by High‐Resolution Mass Spectrometry

Abstract

DNA alkylation: The reactivity and selectivity of DNA-alkylating duocarmycin analogues were determined by direct ESI-FTICRMS (negative-ion mode) measurements. The high efficiency of a new seco drug for the alkylation of duplex DNA has been determined in this way for the first time (picture: drug bound covalently to an oligodeoxynucleotide). The corresponding, less toxic prodrug and the enantiomer of the seco drug show, in contrast, only a very low alkylation tendency.