Discrimination of the Hoechst side population in mouse bone marrow with violet and near‐ultraviolet laser diodes

Abstract

These evaluations indicated that near-UV laser diodes give excellent Hoechst SP resolution on cuvette-based instruments. As the next generation of cell sorters integrate cuvette-based cell interrogation into conventional jet-in-air cell separation, these laser sources should become applicable for analysis and physical separation of Hoechst SP cells.