Implementation of PCB-Based PCR Chip Using Double-Sided Tape

Abstract

The fabrication of microfluidic channels in previously proposed polymerase chain reaction chips by using polydimethylsiloxane, polycarbonate, and poly (methyl methacrylate) is expensive. Hence, the channel fabrication using tapes was investigated in this study. This paper presents experimental results for DNA amplification performance and the adsorption of fluorescent substances with respect to various taping materials constituting microfluidic channels. Experiments on fluorescence adsorption showed the adhesive of double-sided tapes was the main cause of the adsorption. DNA amplification with microfluidic channels made of polypropylene and the double-sided tape showed comparable performances to that with the conventional tube in the general thermal cycler.