Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans

Abstract

A Gram-stain-negative, facultatively anaerobic, gliding, yellow-pigmented bacterium, designated SS12(T), was isolated from shark gill homogenate and characterized using a polyphasic approach. The strain was catalase-positive and oxidase-negative. Optimal growth occurred at 28-30 °C, pH 7.0-7.5 and in the presence of 2-4% (w/v) NaCl. The DNA G+C content was 40.0 mol%. The strain contained MK-7 as the prevailing menaquinone; iso-C15 : 0 and anteiso-C15 : 0 as the major cellular fatty acids; and phosphatidylethanolamine and an unknown lipid as the predominant polar lipids. Comparative analysis of 16S rRNA gene sequences demonstrated that the novel isolate showed the highest sequence similarity (94.68%) to Saccharicrinis fermentans DSM 9555(T) and the sequence similarities among the type strains of all other species studied were less than 92%. A phylogenetic tree, based on 16S rRNA gene sequences, showed that strain SS12(T) and Saccharicrinis fermentans DSM 9555(T) formed a distinct cluster within the family Marinilabiliaceae. On the basis of its phylogenetic position and phenotypic traits, strain SS12(T) represents a novel species of genus Saccharicrinis, for which the name Saccharicrinis carchari sp. nov. is proposed. The type strain is SS12(T) ( = CICC 10590(T) = DSM 27040(T)). Emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans are also provided.