Electroporation system generating wide range square-wave pulses for biological applications

Abstract

The design of a wide range square-wave pulse electroporation system is presented. The system is able to generate pulses having voltage amplitudes up to 4000 V and currents up to 100 A. The quantity of accumulated energy is optimized by the use of the variable capacitor bank. The proposed device has an integrated graphic LCD and measurement modules for current pulse visualization, and does not require an external oscilloscope. The whole system was tested with suspension of human lymphoma cells of Jurkat line in RPMI medium under a variety of electroporation conditions.