Microbacterium panaciterrae sp. nov., isolated from the rhizosphere of ginseng

Abstract

Strain DCY56(T) was isolated from a soil sample taken from a ginseng field. The strain was Gram-reaction positive, catalase-positive, oxidase-negative, aerobic and non-motile. Phylogenetic analysis, based on 16S rRNA gene sequence analysis, indicated that strain DCY56(T) belonged to the genus Microbacterium. The closest relatives were Microbacterium azadirachtae AI-S262(T), Microbacterium aerolatum V-73(T) and Microbacterium phyllosphaerae DSM 13468(T) (98.0 %, 98.0 % and 97.5 % gene sequence similarity, respectively). The G+C content of the genomic DNA of strain DCY56(T) was 68.5 mol%. The DNA-DNA relatedness values between strain DCY56(T) and the most closely related type strains were lower than 36 %. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and an unidentified glycolipid. The predominant fatty acids contained iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The menaquinones were MK-12 and MK-13. The diagnostic diamino acid of strain DCY56(T) was ornithine. The dominant whole-cell sugars were glucose, rhamnose and ribose. The results of the genotypic analysis, in combination with chemotaxonomic and physiological data, demonstrate that strain DCY56(T) represents a novel species within the genus Microbacterium, for which the name Microbacterium panaciterrae sp. nov. is proposed. The type strain is DCY56(T) ( = KCTC 19884(T) = JCM 17839(T)).