Directed Evolution of an Amine Oxidase for the Preparative Deracemisation of Cyclic Secondary Amines

Abstract

Off the rac. Directed evolution of the amine oxidase from Aspergillus niger (MAO-N), by using 1-methyltetrahydroisoquinoline as the target substrate, led to the identification of a variant enzyme (Asn336Ser/Ile246Met) that possessed a greater kcat value (5.5-fold) than the parent as well as high enantioselectivity (see scheme). This new variant was used for the deracemisation of cyclic secondary amines on a preparative scale. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2268/2005/z400329_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.