Memórias do Instituto Oswaldo Cruz · 1996 · 19 citations · 14 references
Life SciencesBiologyMicrobiologyMycologyParasitologyProtozoologyBlood ParasitesChagas DiseaseParasitic PathogenesisParasitic ProtozoaProtozoal InfectionsProtozoan Host-parasite InteractionsAxenic Culture EstablishmentAxenic Culture MaintenanceBlood Culture TechniquesComparative Growth AnalysisPopulation SizePopulation Size MeasurementTrypanosomatid-host InteractionsTrypanosome Infections
Trypanosoma minasense was isolated for the first time in blood axenic culture from a naturally infected marmoset, Callithrix penicillata, from Brazil. The parasite grew profusely in an overlay of Roswell Park Memorial Institute medium plus 20% foetal bovine serum, on Novy, McNeal and Nicolle medium (NNN), at 27 degrees C, with a peak around 168 hr. The morphometry of cultural forms of T. minasense, estimates of cell population size and comparative growth in four different media overlays always with NNN, were studied. The infectivity of cultural forms to marmosets (C. penicillata and C. jacchus) and transformation of epimastigotes into metacyclic-like forms in axenic culture in the presence of chitin derivates (chitosan) were evaluated.
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John O. Corliss, Cecil A. Hoare · Transactions of the American Microscopical Society · 1973 · 532 citations
Dennis M. Dwyer · American Journal of Tropical Medicine and Hygiene · 1977 · 499 citations