Publication | Open Access
An Improved Method for the Demonstration of the <I>in situ</I> Chloroplast Nuclei in Higher Plants
114
Citations
4
References
1980
Year
Improved MethodNuclear StructureBotanyMolecular BiologyChloroplast NucleiPlant PathologyPlant DevelopmentPlant Molecular BiologyHigher PlantsVicia FabaPhotosynthesisPlant CytologyHealth SciencesPlant BiologyFluorescent Light MicroscopyPhotosystemsSpherical Ct-nucleiPlant HistologyBiologyNatural SciencesPlant Physiology
A 4'6-diamidino-2-phenyindole (DAPI) staining technique was devised to demonstrate the in situ chloroplast nuclei (ct-nuclei) in cells of higher plants. Fluorescent light microscopy of samples stained by this method revealed that most mature chloroplasts of Vicia faba contained about 15-30 small, spherical ct-nuclei.
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