Proceedings of the National Academy of Sciences · 1995 · 116 citations · 28 references
Life SciencesBiochemistryMolecular BiologyGene ExpressionGene RegulationTranscriptional RegulationHormone ReceptorsHormone SignalingLigand BindingNuclear ReceptorsTranscription FactorsTranscriptional ControlBasal Transcription MachineryBasal Transcriptional MachineryLigand-dependent RegulationNuclear Hormone ReceptorsRetinoid ReceptorsRetinoid X ReceptorsTata-binding Protein FunctionTranscriptional Activation By Hormones
The retinoid X receptor (RXR) participates in a wide array of hormonal signaling pathways, either as a homodimer or as a heterodimer, with other members of the steroid and thyroid hormone receptor superfamily. In this report the ligand-dependent transactivation function of RXR has been characterized, and the ability of RXR to interact with components of the basal transcription machinery has been examined. In vivo and in vitro experiments indicate the RXR ligand-binding domain makes a direct, specific, and ligand-dependent contact with a highly conserved region of the TATA-binding protein. The ability of mutations that reduce ligand-dependent transcription by RXR to disrupt the RXR-TATA-binding protein interaction in vivo and in vitro suggests that RXR makes direct contact with the basal transcription machinery to achieve activation.
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A novel genetic system to detect protein–protein interactions
Stanley Fields, Ok-Kyu Song · Nature · 1989 · 6.1K citations