A highly sensitive long-wavelength fluorescence probe for nitroreductase and hypoxia: selective detection and quantification

Abstract

A novel long-wavelength fluorescence probe has been developed for the detection of nitroreductase (NTR) and hypoxia. could be activated by NTR at 0.1 μM to release the fluorophore and significant changes in fluorescence emission at 658 nm were observed. This feature makes it advantageous for imaging hypoxic cells with minimal endogenous interference.