A versatile primer for DNA sequencing in the M13mp2 cloning system

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.3K citations · Full text

Life Sciences, Microbiology, Molecular Biology +10

Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of <i>Escherichia coli</i> by R-Factor DNA

Stanley N. Cohen, Annie Chang, Leslie Hsu · Proceedings of the National Academy of Sciences · 1972 · 2.7K citations

Life Sciences, Genetics, Microbiology +15

The use of thin acrylamide gels for DNA sequencing

Frederick Sanger, Alan Coulson · FEBS Letters · 1978 · 1.4K citations

Life Sciences, Genetics, Molecular Biology +8

Construction and characterization of new cloning vehicles III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules

Francisco Bolívar · Gene · 1978 · 748 citations

Life Sciences, Biotechnology, Molecular Biology +17

Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.

Joachim Messing, Bruno Gronenborn, Benno Müller‐Hill et al. · Proceedings of the National Academy of Sciences · 1977 · 708 citations · Full text

Life Sciences, Microbiology, Molecular Biology +16