Nocardioides paucivorans sp. nov. isolated from soil

Abstract

One strain, designated KIS31-44(T), was isolated from a soil sample collected from Dokdo Island, South Korea. The strain is Gram-stain-positive, aerobic, non-spore-forming and non-motile. It grows optimally at 28-30°C, at pH 7.0 and 0% NaCl. 16S rRNA gene sequence analysis showed that strain KIS31-44(T) belonged to the genus Nocardioides and shared the highest sequence similarities with Nocardioides aestuarii JC2056(T) (95.5%) and Nocardioides terrae VA15(T) (95.0%). The major fatty acids of strain KIS31-44(T) were C(17:1) ω6c, C(18:1) ω9c, summed feature 3 (C(16:1) ω6c and/or C(16:1) ω7c), iso-C(16:0), C(18:0) 10-methyl (TBSA), C(16:0) 2-OH, C(17:0) 10-methyl, and iso-C(16:1) H. The major isoprenoid quinone was MK-8 (H₄). The strain contained diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as the major polar lipids. The peptidoglycan structure was A3γ-type with LL-diaminopimelic acid. Based on these data, the isolate represents one novel species in the genus Nocardioides, for which the name Nocardioides paucivorans sp. nov. (type strain KIS31-44(T) =DSM 27142(T) =KACC 17309(T)) is proposed.