In vitro replication of the<i>Cydia pomonella</i>(codling moth) granulosis virus

Abstract

Nuclear polyhedrosis virus (family Baculoviridae, subgroup A) in vitro systems have been well established for more than ten years, and have permitted application of modern microbiological techniques. Although widely tried, similar systems for granulosis virus (subgroup B) replication have not been obtained to our knowledge. We report here on the successful in vitro replication of Cydia pomonella granulosis virus in a Cydia pomonella primary cell line. The replication was confirmed by light and electron microscopy. This granulosis virus in vitro replication system will be an important tool for further granulosis virus research and for comparative studies on granulosis and nuclear polyhedrosis virus replication.