THE FEMALE SEX HORMONE

Abstract

Since our demonstration 1 of the female sex hormone in the circulating blood in August, 1925, it has been our aim to simplify the technic to such an extent as to enable any well equipped laboratory to perform the test. It appears to us that we have now succeeded in so doing. For details of methods previously described, the reader is referred to papers VI 2 and VII, 3 which appeared inThe Journalin 1926 and 1927. Our present technic enables us to prepare the blood for injection in the course of two and one-half hours without the aid of any complicated apparatus or methods. As numerous investigators, including ourselves, have found that individual mice show a greatly varying susceptibility to the reaction, we deem it safer to abstract 40 cc. of blood in order that a higher dose than a single mouse unit (M. U.) should be available