Measurement of Serum Haptoglobin in Neonatal Farm‐Raised and Bob Veal Calves Using Two Immunoassay Methods

Abstract

Two immunoassays using an anti-bovine haptoglobin monoclonal antibody, Hap 1, were used to measure serum haptoglobin levels in neonatal farm-raised and bob veal calves. Bob veal calves were grouped into condemned, normal, and icteric groups based on the appearance of the carcass and viscera at postmortem examination. The competitive inhibition assay was more sensitive than the direct hemoglobin binding assay in detecting low levels of haptoglobin in all groups of calves. A significant number of bob veal calves with gross postmortem lesions other than icterus had detectable haptoglobin levels. The low levels of haptoglobin that were detected were not useful in distinguishing the relative severity of the inflammatory or degenerative process; however, the predictive values of a positive and negative test suggest haptoglobin measurement may be useful as a supplemental tool in evaluating the health status of the neonatal calf.