On the Activation of Fructose‐1,6‐bisphosphatase of Spinach Chloroplasts and the Regulation of the Calvin Cycle

Abstract

Activation by light of spinach fructose-1,6-bisphosphatase is mimicked by dithiothreitol. This process of activation by dithiothreitol implies the specific reduction of two disulfide bridges and a conformation change of the enzyme that makes eight sulfhydryl groups available. The activated enzyme has an apparent allosteric kinetic behavior with respect to both fructose 1,6-bisphosphate and magnesium.