Hydrogel-coated textile scaffolds as candidate in liver tissue engineering: II. Evaluation of spheroid formation and viability of hepatocytes

Abstract

Abstract In this study we evaluate the performance of primary rat hepatocytes and HepG2 cells on chitosan-collagen hydrogel-coated textile scaffolds. Light microscopy and electron microscopic observations showed attachment and aggregate formation tendency of hepatocytes on the scaffolds. As tested by the tetrazolium reduction (MTT) assay it was evident that cells had preserved mitochondrial functionality. It was also observed that pure collagen and collagen blended scaffolds allowed higher cell growth than pure chitosan scaffold. Fluorescent live/dead staining showed a metabolically active, viable cell population on all scaffold compositions with occurrence of few dead cells. Cell functionality was confirmed by secretion of albumin, which was maintained throughout culture period. Take collectively our results suggests that hydrogel-coated textile scaffolds could be promising for tissue-engineering applications, as they allow favorable hepatocyte attachment, spheroid formation and maintenance of function. These scaffolds could be useful for co-culturing hepatocytes and nonparenchymal endothelial cells in bioartificial liver support systems. Keywords: CHITOSANCOLLAGENPETHEPATOCYTESDIFFERENTIATIONTISSUE ENGINEERING.