The kinetic and mass transfer behavior of immobilized invertase on ion‐exchange resin beads

Abstract

Abstract Immobilized invertase was prepared by binding native invertase to a polyamine type ion‐exchange resin. Kinetic behavior of the immobilized invertase on these small pellets was investigated in a packed‐bed reactor. For low flow rates, an effect of interparticle diffusion on the Machaelis constant was observed and a correlation was proposed to evaluate the effect. At high flow rates, Michaelis and inhibition constants were determined and compared with those for native invertase. With large pellets, intraparticle diffusion was found to be important and at high substrate concentrations, the effectiveness factor exceeded unity. Good agreement was found between the theoretical analyses and the experimental data.