Nutrition of Animal Cells in Tissue Culture. I. Initial Studies on a Synthetic Medium.,

Abstract

SummaryThe nutrition of animal cells in tissue culture has been studied with particular reference to amino acids, vitamins, nucleic acid constituents and various accessory growth factors. The optimal concentrations of these substances for the maintenance of cell life in vitro have been established and a completely synthetic feeding solution has been devised. Although this mixture will not support cell life indefinitely, it has been found adequate to maintain chick embryo cells for an average period of 4 to 5 weeks. The mixture is being used as a basal synthetic medium for further studies on the unidentified growth-promoting substances known to occur in natural media.