Nucleosides and Nucleotides · 1996 · 13 citations · 31 references
Veterinary MedicineInfectious DiseasesMolecular BiologyDiagnostic ParasitologyMolecular DiagnosticsParasitologyBlood Parasite DiagnosisBlood ParasitesMalariaMedical ParasitologyMolecular DiagnosisMolecular Diagnosis Of ParasitesAcridine Orange TestAssayColorimetric TestsDetection Of Protozoan ParasitesMalaria ParasitesMolecular Detection Of ParasitesPlasmodium FalciparumPlasmodium Vivax
Abstract We have developed a colorimetric assay, “microtiter plate-hybridization”, for the detection of malaria parasites Plasmodium falciparum and P. vivax in human blood, in which the target DNA sequences (18s small subunit ribosomal RNA gene) amplified by polymerase chain reaction (PCR) are hybridized with the species-specific probes immobilized on a microtiter well. This assay system was tested in Guadalcanal, Solomon Islands, where malaria is highly endemic. We obtained blood samples by finger puncture from 130 asymptomatic donors. Among the 130 samples, 30 (23 %) were P. falciparum positive, 28 (22 %) were P. vivax positive, and 8 (6 %) were mixed infections. The results of our DNA diagnostic method showed good correlation with those of acridine orange microscopy.
31
Human Malaria Parasites in Continuous Culture
William Trager, James B. Jensen · Science · 1976 · 8K citations
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
RK Saiki, DH Gelfand, Susanne Stoffel et al. · Science · 1988 · 4.3K citations
Georges Snounou, Suganya Viriyakosol, William Jarra et al. · Molecular and Biochemical Parasitology · 1993 · 919 citations