Applications of isothermal titration calorimetry to measure enzyme kinetics and activity in complex solutions

Estimation of macromolecule concentrations and excluded volume effects for the cytoplasm of Escherichia coli

Steven B. Zimmerman, Stefan O. Trach · Journal of Molecular Biology · 1991 · 1.4K citations

Life Sciences, Biophysics, Microbiology +13

Protein-solvent preferential interactions, protein hydration, and the modulation of biochemical reactions by solvent components

Serge N. Timasheff · Proceedings of the National Academy of Sciences · 2002 · 707 citations

Natural Sciences, Biochemistry, Chemistry +17

Vapor Pressure Osmometry Studies of Osmolyte−Protein Interactions:  Implications for the Action of Osmoprotectants in Vivo and for the Interpretation of “Osmotic Stress” Experiments in Vitro

Elizabeth S. Courtenay, Charles Anderson, M. Thomas Record · Biochemistry · 2000 · 431 citations

Natural Sciences, Biochemistry, Biophysics +17

Enzyme Kinetics Determined Using Calorimetry: A General Assay for Enzyme Activity?

Matthew J. Todd, Javier Gómez · Analytical Biochemistry · 2001 · 346 citations

Life Sciences, Biochemistry, Chemical Kinetics +16

Effect of macromolecular crowding upon the structure and function of an enzyme: glyceraldehyde-3-phosphate dehydrogenase

Allen P. Minton, Jacob Wilf · Biochemistry · 1981 · 320 citations

Life Sciences, Biochemistry, Molecular Biology +17