Integrin targeting using RGD‐PEI conjugates for <i>in vitro</i> gene transfer

Abstract

Coupling of the tetrapeptide RGDC without a PEG spacer improved transfection efficiency of PEI in integrin-expressing Mewo cells by 1-2 orders of magnitude, especially at low N/P ratios. The use of a PEG spacer seems to impair targeting, possibly by not only shielding PEI, but also the RGD ligand.