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23. Immunization, Isolation of Immunoglobulins, Estimation of Antibody Titre
860
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1973
Year
AllergyMedicineImmunologyVeterinary ScienceAntibody ScreeningImmunochemistryHumoral ImmunityAntibody EngineeringAntibody TitreImmunoglobulin EImmunotherapyFinally Antibody TitresIon Exchange ChromatographyAnti‐human IggAntibody Biology
The study details an immunization schedule and a protocol for isolating immunoglobulins from antiserum using salting out and ion exchange chromatography. High‑titer monospecific antibodies were produced in rabbits with as little as 25 µg antigen per kg, while polyspecific antibodies required a ten‑fold higher dose; antibody titres were then compared across four titration methods. Summary.
Summary. High titred monospecific antibodies are obtained in rabbits injecting as little as 25 μg pure antigen per kg body weight. For polyspecific antibodies the dose is increased 10 times. The immunization schedule is described in detail. A procedure is given for the isolation of the immunoglobulins from antiserum by salting out and ion exchange chromatography. Finally antibody titres as measured by 4 different titration methods are compared using anti‐human IgG from rabbits as a model.