Immbilization on cellulose in bead form after periodate oxidation and reductive alkylation

Abstract

The covalent binding of trypsin to cellulose beads after periodate oxidation was examined. The degree of cellulose derivatives solubilization is directly related to cellulose oxidation and increases with the increasing pH of the reaction mixture. The quantity of trypsin immobilized was examined as a function of pH, reaction time, and concentration of native trypsin in the reaction mixture during the coupling reaction. The catalytic activity and the stability of the preparations of immobilized trypsin during continuous hydrolysis of low-molecular-weight and high-molecular-weight substrates was assayed.