PHOTO-CONTROL OF ENZYME ACTIVITY WITH A PHOTOCHROMIC SPIROPYRAN COMPOUND —MODIFICATION OF α-AMYLASE WITH SPIROPYRAN COMPOUND—

Abstract

Abstract A photochromic α-amylase was prepared by chemical modification with a spiropyran compound and isolated with DEAE-Sephadex chromatography. The modified α-amylase showed the reverse photochromism in water. UV- or VS-light irradiation caused a decrease in enzyme activity of the modified enzyme. The initial activity could be recovered when the modified enzyme irradiated was kept in the dark.