DNA‐RFLP METHODS and INTERPRETATION SCHEME FOR HLA‐DR and DQ TYPING

Abstract

DNA-restriction fragment length polymorphism (RFLP) typing is a well established and standard technique for identification of HLA-DR and DQ allotypes. We present a detailed review of methods for RFLP analysis and a scheme for interpretation of results. This scheme permits the routine identification of HLA-DR and DQ allotypes using the restriction endonucleases TaqI and either HindIII or MspI.