Acridone-labeled Base-discriminating Fluorescence (BDF) Nucleoside: Synthesis and Their Photophysical Properties

Abstract

Abstract Acridone-labeled BDF bases AcA and AcU were incorporated into oligonucleotides (ODNs) and their photophysical properties were evaluated. The BDF probe containing AcA is extremely powerful in recognizing opposite base T via enhancement of the fluorescence intensity. Furthermore, they emit strong fluorescence at a longer wavelength than previously reported pyrene-labeled BDF probes and thus can be used for the detection of SNPs.