Characterization of Rh Antibodies Formed after Incompatible Pregnancies and after Repeated Booster Injections

Abstract

Abstract. Indirect hemagglutination studies were performed on 10 incomplete anti‐Rh sera from mothers after incompatible pregnancies, on 19 incomplete anti‐Rh plasma samples from preimmunized and repeatedly boosted individuals and on 3 anti‐Rh plasma pools. Group O Rh‐positive red blood cells were agglutinated by antisera to immunoglobulins. Anti‐Rh antibodies after incompatible pregnancies were found to belong to the immunoglobulin class IgG and to the subclasses IgG1, IgG3 and IgG4 only. In most anti‐Rh samples from preimmunized and repeatedly boosted subjects, IgG and IgA were present. In six anti‐Rh samples of this group, the subclass IgG2 could be demonstrated.