Determination of<i>Pseudomonas Putida</i>Live Cells with Classic Cultivation and Staining with “Live/Dead Baclight Bacterial Viability Kit”

Abstract

ABSTRACTPeriodic culture of Pseudomonas putida strain ATCC 12633=NBPMCC1090 was investigated in a 24 h experiment for sensitivity to zinc oxide thin films constructed under different conditions. The growth of bacteria was followed at every three hours and three methods were used to determine live cells. Optical density, cultivation and microscopic methods were applied and compared. To distinguish active and inactive cells LIVE/DEAD BacLight Bacterial Viability Kit staining was compared with classical cultivation methods. Bacterial quantity determinated on nutrient media, appear higher than those of live cells counted on epifluorescent microscope.Keywords: epifluorescent microscopeLIVE/DEAD BacLight Bacterial Viability Kitpropidium iodide (PI)Pseudomonas putidazinc oxide thin films