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Extending homologous sequence based on the single gene mutants by one-step PCR for efficient multiple gene knockouts

Single Gene MutantsEngineeringOne-step PcrMolecular Biological MethodDna ReplicationSynthetic BiologyMolecular BiologyGenetic EngineeringNucleic Acid AmplificationSynthetic CircuitHomologous SequenceSystems BiologyMedicineBioinformaticsGenome Editing