Cryopreservation of Yellow Perch Semen

Abstract

The objective of this study was to investigate methods for the cryopreservation of yellow perch (Perca flavescens) semen. Two-year-old males were induced to spermiate 8–10 weeks before natural spawning by using an analog of luteinizing hormone releasing hormone (LHRH). Milt was cryopreserved with either 0.3 M glucose–20% glycerol or an extender containing 8% dimethyl sulfoxide (DMSO) supplemented with 10% egg yolk. Samples were frozen on dry ice and stored in liquid nitrogen. Spermatozoa frozen in the DMSO extender had a fertilization rate of 23.2%, significantly higher than obtained with glucose–glycerol (0.3%) but less than obtained with fresh semen (53.4%).