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THE SECRETION OF IMMUNO-REACTIVE LUTEINIZING HORMONE AND FOLLICLE-STIMULATING HORMONE BY THE HUMAN FOETAL PITUITARY IN ORGAN CULTURE
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0
References
1971
Year
FertilityHuman GrowthGynecologyFemale Reproductive FunctionReproductive BiologyEmbryologyReproductive EndocrinologyEmbryo CulturePituitary GlandReproductive MedicinePituitary DiseasePublic HealthGrowth HormoneEndocrine MechanismDevelopmental EndocrinologyEndocrinologyPlacental FunctionDevelopmental BiologyPhysiologyReceptor BiologyRadioactive GlucosamineTissue CultureMedicineReproductive Hormone
SUMMARY Foetal human pituitaries of 8–18 weeks gestational age were maintained in tissue culture for up to 6 days. No morphological change was observed in the tissue during this period. Radioactive glucosamine was continuously incorporated into medium protein and the levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH) and growth hormone in the medium increased with the duration of the culture. Theophylline stimulated the release of both LH and FSH consistently over the 6 days, and the total amount of each hormone released over this period was significantly greater than that in unstimulated control cultures. Cyclic adenosine 3′,5′-monophosphate stimulated FSH release to some extent but had no significant effect on LH release.