Freezing of Bovine Semen. II. Effect of Milk Solids Level, Glycerol Level, and Fructose on Freezability of Bull Spermatozoa in Reconstituted and Fresh Skimmilk Diluents

Abstract

This investigation revealed that the level of glycerol required for optimum spermatozoan freezability was related to the total solids content of skimmilk diluents, and that 1.25% added fructose improved the post-thawing motility of spermatozoa frozen in reconstituted skimmilk. Reconstituted skimmilk gave optimum spermatozoan survival when the glycerol percentage by volume equalled the total solids l~ercentage by weight, and when both were 9 or 11%. In fresh skimmilk containing 9% solids, 11% glycerol was significantly better than 9 or 13%, Editor.