Measurement of activity of peroxidase isoenzymes in flax (<i>Linum usitatissimum</i>)

Abstract

Peroxidase isoenzymes may be separated on acrylamide gels and then detected by supplying the substrate in an appropriate reaction system. One such system frequently used contains guaiacol as the hydrogen donor, although this compound has certain drawbacks. Ways of circumventing these drawbacks are suggested, so that quantitative estimates of the activity of individual peroxidase isoenzymes may be obtained.