Determination of Biological Oxidative Stress Using High Performance Liquid Chromatography with Electrochemical Detection (HPLC-ECD)

Abstract

For three decades, high performance liquid chromatography has proven itself to be a powerful, flexible, and inexpensive tool for basic and clinical research. Recent advances in our understanding of disease have prompted a demand for more sensitive and selective methods of routine bioanalysis, particularly with respect to the determination of oxidative metabolites and biomarkers of oxidative stress. Multidimensional detectors utilizing coulometric arrays offer a solution to these research needs. The challenge to the bioanalyst is now to creatively apply HPLC-ECD technology to promising research and clinical enterprises.