Membrane‐associated phosphoinositidase C activity in <i>Dictyostelium discoideum</i>

Abstract

Membrane-associated phosphoinositidase C activity has been identified in Dictyostelium discoideum using phosphatidylinositol 4,5-bisphosphate as exogenous substrate. Maximal activity was observed with 0.4 mM phosphatidylinositol 4,5-bisphosphate at pH 7.0. The enzyme was stimulated by micromolar concentrations of free calcium with maximal activity at 100 microM.