Zinc‐binding and structural properties of the histidine‐rich loop of <i>Arabidopsis thaliana</i> vacuolar membrane zinc transporter MTP1

Abstract

The vacuolar Zn(2+)/H(+) antiporter of Arabidopsis thaliana, AtMTP1, has a cytosolic histidine-rich loop (His-loop). We characterized the structures and Zn(2+)-binding properties of the His-loop and other domains. Circular dichroism analyses revealed that the His-loop partly consists of a polyproline type II structure and that its conformational change is induced by Zn(2+) as well as the C-terminal domain. Isothermal titration calorimetry of the His-loop revealed a binding number of four Zn(2+) per molecule. Numbers of Ni and Co associated with the His-loop were approximately one ion per molecule and the thermodynamic parameters of the association with these ions were different from that of Zn(2+). These results suggest the involvement of the His-loop in sensing cytosolic Zn(2+) and in the regulation of zinc transport activity through Zn(2+)-induced structural change.