Biochemical Changes in Liver and Blood During Liver Fattening in Rats

Abstract

Excessive fat accumulation in the liver is a common metabolic disorder seen in humans and animals.Fatty liver was induced in the rat by feeding the animals witfr a sucrose rieh diet containing l % orotic acid for 2-3 weeks.In the sera from fatty liver rats there were significant changes in the level of alanine aminotransferase (+ 68.7%), malic dehydrogenase (+ 77.8%), -glutamyl transpeptidase (-53.4%) and total lipids (+ 26.6%).There were small to no changes in the levels of aspartate aminotransferase, glucose-6-phosphate dehydrogenase, lactic dehydrogenase, aldolase, malic enzyme, 6-phosphogluconic acid dehydrogenase, alka-[ | line phosphatase and albumin.| In fatty liver, significant differences were seen in the levels of glucose 6-phosphate dehydrogenase (-f 235%), l * malic enzyme (+ 170%), -glutamyl transpeptidase (+ 113%), 6-phosphogluconate dehydrogenase (+ 63%), aspartate aminotransferase (+ 35.6%), malic dehydrogenase (+ 38%), lactic dehydrogenase (+ 37%), and alanine aminotransferase (-23%).Comparison of the non-fatty part with the fatty part of the fatty liver showed larger changes in the nonfatty part of the liver, suggesting that during the fattening process, there is an induction of enzymes in the liver reaching a peak prior to lipid accumulation, declining thereafter during liver fattening.The increase in NADPH-generating lipogenic enzymes suggests that accumulated fat in the liver is at least partially from ! de^-novo increased synthesis in the liver.i nderungen biochemischer Kenngren in Leber und Blut whrend Leberverfettung bei der Ratte Zusammenfassung: Die exzessive Anhufung von Fett in der Leber ist eine bei Mensch und Tier beobachtete Stoffwechselstrung. Durch Verftterung einer 1% Orotsure enthaltenden Saccharose-reichen Dit fr 2-3 Wochen wurde bei Ratten eine Fettleber hervorgerufen.Im Serum der Ratten mit Fettleber waren signifikant verndert: Alaninaminotransferase (+ 68,7%), Malatdehydrogenase (+ 77,8%), -Glutamyltransferase (-53,6%) und Gesamt-Lipide (+ 26,6%); gering oder nicht verndert waren: