Publication | Closed Access
AN IN VIVO TEST FOR THE IMMUNOCOMPETENCE OF HUMAN LYMPHOCYTES
11
Citations
0
References
1973
Year
Cell TherapyLaboratory ImmunologyImmunodeficienciesImmunologyImmunoeditingPathologyImmunophenotypingImmunotherapyHematologyLymphatic SystemLaboratory MedicineCell TransplantationCancer ResearchMedium 199Recipient MiceLymphoid NeoplasiaAutoimmune DiseaseAutoimmunityTumor MicroenvironmentUrologyMedicineReaction Size
After the injection of mice with human lymphocytes suspended i.d. in Medium 199 a local area of induration was obtained. This was maximal at 48 hr and correlated to the number of lymphocytes injected. Evidence that this normal lymphocyte transfer reaction was graft-versus-host in character was the finding that the reaction size was independent of the administration of rabbit antimouse thymocyte serum to the recipient mice. However, the reaction could be suppressed by administration of rabbit antihuman lymphocyte serum. Lymphocytes from 7 of 10 patients with advanced carcinoma of the urinary bladder, 4 patients with advanced carcinoma of the breast, and 1 case each of carcinoma of the prostate, carcinoma of the uterus, and malignant melanoma showed diminished normal lymphocyte transfer reactivity. This was assessed both in relation to patients of similar age with debilitating nonneoplastic conditions and to patients in a surgical ward whose general condition was good.