Science · 1991 · 389 citations · 46 references
Life SciencesBiochemistryMolecular BiologyGene ExpressionMolecular VirologyVirologyRna TranscriptionRna-binding ProteinsRna-protein InteractionsViral Regulatory ElementsViral Rna BiologyViral TranscriptionRetroviral TranscriptionRna-binding Protein InteractionsTrans-acting Rna-binding ProteinsViral Rna TranscriptionViral Transactivators
Human immunodeficiency virus type 1 (HIV-1) gene expression is activated by Tat, a virally encoded protein. Tat trans-activation requires viral (trans-activation--responsive; TAR) RNA sequences located in the R region of the long terminal repeat (LTR). Existing evidence suggests that Tat probably cooperates with cellular factors that bind to TAR RNA in the overall trans-activation process. A HeLa complementary DNA was isolated and characterized that encodes a TAR RNA-binding protein (TRBP). TRBP activated the HIV-1 LTR and was synergistic with Tat function.
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Anti-termination of transcription within the long terminal repeat of HIV-1 by tat gene product
Shaw-Yi Kao, Andrew F. Calman, Paul A. Luciw et al. · Nature · 1987 · 908 citations