The agarose diffusion method for ocular irritancy screening: cosmetic products, part ii

Abstract

Twenty-two cosmetic products were tested by the agarose diffusion method to determine the correlation between the zone of lysis produced by agarose diffusion and the 24 hr rating of chemical irritation in previously conducted Draize eye irritancy tests using these same formulations. The cosmetic product formulation types included a shaded range of water-in-oil emulsions, water-based or hydrocarbon-based suspensions, gels, and physical mixtures of powders or waxes. A 100% correlation was established for this group of samples between the in vitro agarose diffusion test and the in vivo Draize eye irritancy test, with no false-positive or false-negative results.