On the Origin of the Low-Spin Character of Cytochrome P450cam in the Resting State—Investigations of Enzyme Models with Pulse EPR and ENDOR Spectroscopy

Abstract

The P450 enzyme model 1 is a high-spin system. EPR and ENDOR spectra reveal the coordination of water to the Fe^III center. This is the first experimental proof that coordination of water is not the single determining factor in the stabilization of the low-spin character of the cytochrome P450 resting state.