Target‐Assisted Selection of Galactosyltransferase Binders from Dynamic Combinatorial Libraries. An Unexpected Solution with Restricted Amounts of the Enzyme

Abstract

Fix and look: Dynamic combinatorial chemistry coupled to an irreversible transformation has been used to identify enzyme binders in a library directed towards an α-1,3-galactosyltransferase. Small amounts of the protein induce an accumulation of the nonbinding amine products such as A resulting from the reduction of the corresponding imine binders. The binding properties of the imines are best mimicked by amides such as B. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2268/2006/z600022_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.