Physical Review Letters · 2006 · 225 citations · 22 references
EngineeringMicroscopyExtracellular MicrovesiclesCytoskeletonCell BiophysicsBiomedical EngineeringCell MechanicsCellular PhysiologyMembrane FluctuationsCell Membrane TensionMatrix BiologyLight MicroscopyBiophysicsCell BiomechanicsOptical InterferometryBiophotonicsCell BiologyBiophysical AspectCellular BiochemistryMedicineRed Blood Cells
The cytoskeleton confinement model explains that the cytoskeleton suppresses membrane fluctuations. A novel noncontact optical interferometry method measures nanoscale thermal fluctuations in red blood cells and giant unilamellar vesicles. The measurements reveal that red blood cells exhibit a nonzero tension that increases from discocytic to spherical shapes, while giant vesicles show 4–24 times lower tension, yet both share similar bending moduli. Published in Phys.
Using a novel noncontact technique based on optical interferometry, we quantify the nanoscale thermal fluctuations of red blood cells (RBCs) and giant unilamellar vesicles (GUVs). The measurements reveal a nonvanishing tension coefficient for RBCs, which increases as cells transition from a discocytic shape to a spherical shape. The tension coefficient measured for GUVs is, however, a factor of 4-24 smaller. By contrast, the bending moduli for cells and vesicles have similar values. This is consistent with the cytoskeleton confinement model, in which the cytoskeleton inhibits membrane fluctuations [Gov et al., Phys. Rev. Lett. 90, 228101, (2003).
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Laser phase and frequency stabilization using an optical resonator
R. W. P. Drever, J. L. Hall, Frank V. Kowalski et al. · Applied Physics B · 1983 · 3.7K citations